peptide side-chain mimetics Search Results


peptoids  (Mimetics)
86
Mimetics peptoids
Peptoids, supplied by Mimetics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peptide+side-chain+mimetics/peptoids/10__1161_slash_circresaha__110__225003-139-20-22
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two cyclopeptides with a side-chain lactam bridge and additional 3-(1-naphthyl)-alanine substitution (ic and icnal)  (Karebay Inc)
90
Karebay Inc two cyclopeptides with a side-chain lactam bridge and additional 3-(1-naphthyl)-alanine substitution (ic and icnal)
Effects of linear and <t>cyclic</t> <t>peptides</t> in vitro. (A) Cell viability MTT assay in hepatocytes treated with 0.4 mM palmitic acid (PA) with/without peptides (S1, 59 μM; liPS, IC and ICNal, 25 μM). Medium with 10 % FBS was used as positive control. (B) Cell-based ELISA assay for STAT1 activation in hepatocytes stimulated with PA for 6 h in the presence of increasing concentrations of peptides. Values expressed as ratio P-STAT1/STAT1 versus PA alone. (C) NOX-dependent measurement of O2•− in hepatocytes after 16 h of stimulation, assessed by chemiluminescence assay. BSA and apocynin were used as controls. Lucigenin relative units are expressed as fold increases versus basal conditions. (D – F) Expression levels of redox, cell stress, lipid metabolism, and inflammatory genes in hepatocytes pretreated with peptides (S1, 59 μM; liPS, 25 μM; IC/ICNal, 12 and 25 μM) before stimulation with PA for 6 h (D) and 24 h (E and F) . (G) ELISA analysis of cytokine concentrations in hepatocyte conditioned media after 24 h of stimulation. Gene expression of M1 (H) and M2 (I) markers in macrophages incubated for 24 h with conditioned media from hepatocytes stimulated with PA with/without peptides. The qPCR values normalized by 18S are expressed as fold increases versus basal conditions. Results are represented as individual data points and/or mean ± SD of n = 4–6 independent experiments. # P < 0.05 vs basal; ∗P < 0.05 vs PA.
Two Cyclopeptides With A Side Chain Lactam Bridge And Additional 3 (1 Naphthyl) Alanine Substitution (Ic And Icnal), supplied by Karebay Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peptide+side-chain+mimetics/two+cyclopeptides+with+a+side+chain+lactam+bridge+and+additional+3++1+naphthyl++alanine+substitution++ic+and+icnal+/pmc12144417-51-22-40
Average 90 stars, based on 1 article reviews
two cyclopeptides with a side-chain lactam bridge and additional 3-(1-naphthyl)-alanine substitution (ic and icnal) - by Bioz Stars, 2026-09
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biomimetic polymers  (BioMimetic Therapeutics)
90
BioMimetic Therapeutics biomimetic polymers
Effects of linear and <t>cyclic</t> <t>peptides</t> in vitro. (A) Cell viability MTT assay in hepatocytes treated with 0.4 mM palmitic acid (PA) with/without peptides (S1, 59 μM; liPS, IC and ICNal, 25 μM). Medium with 10 % FBS was used as positive control. (B) Cell-based ELISA assay for STAT1 activation in hepatocytes stimulated with PA for 6 h in the presence of increasing concentrations of peptides. Values expressed as ratio P-STAT1/STAT1 versus PA alone. (C) NOX-dependent measurement of O2•− in hepatocytes after 16 h of stimulation, assessed by chemiluminescence assay. BSA and apocynin were used as controls. Lucigenin relative units are expressed as fold increases versus basal conditions. (D – F) Expression levels of redox, cell stress, lipid metabolism, and inflammatory genes in hepatocytes pretreated with peptides (S1, 59 μM; liPS, 25 μM; IC/ICNal, 12 and 25 μM) before stimulation with PA for 6 h (D) and 24 h (E and F) . (G) ELISA analysis of cytokine concentrations in hepatocyte conditioned media after 24 h of stimulation. Gene expression of M1 (H) and M2 (I) markers in macrophages incubated for 24 h with conditioned media from hepatocytes stimulated with PA with/without peptides. The qPCR values normalized by 18S are expressed as fold increases versus basal conditions. Results are represented as individual data points and/or mean ± SD of n = 4–6 independent experiments. # P < 0.05 vs basal; ∗P < 0.05 vs PA.
Biomimetic Polymers, supplied by BioMimetic Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peptide+side-chain+mimetics/biomimetic+polymers/10__1039_slash_d0py01395a-11-32-32
Average 90 stars, based on 1 article reviews
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natural product peptide α-amanitin  (BioMimetic Therapeutics)
90
BioMimetic Therapeutics natural product peptide α-amanitin
Effects of linear and <t>cyclic</t> <t>peptides</t> in vitro. (A) Cell viability MTT assay in hepatocytes treated with 0.4 mM palmitic acid (PA) with/without peptides (S1, 59 μM; liPS, IC and ICNal, 25 μM). Medium with 10 % FBS was used as positive control. (B) Cell-based ELISA assay for STAT1 activation in hepatocytes stimulated with PA for 6 h in the presence of increasing concentrations of peptides. Values expressed as ratio P-STAT1/STAT1 versus PA alone. (C) NOX-dependent measurement of O2•− in hepatocytes after 16 h of stimulation, assessed by chemiluminescence assay. BSA and apocynin were used as controls. Lucigenin relative units are expressed as fold increases versus basal conditions. (D – F) Expression levels of redox, cell stress, lipid metabolism, and inflammatory genes in hepatocytes pretreated with peptides (S1, 59 μM; liPS, 25 μM; IC/ICNal, 12 and 25 μM) before stimulation with PA for 6 h (D) and 24 h (E and F) . (G) ELISA analysis of cytokine concentrations in hepatocyte conditioned media after 24 h of stimulation. Gene expression of M1 (H) and M2 (I) markers in macrophages incubated for 24 h with conditioned media from hepatocytes stimulated with PA with/without peptides. The qPCR values normalized by 18S are expressed as fold increases versus basal conditions. Results are represented as individual data points and/or mean ± SD of n = 4–6 independent experiments. # P < 0.05 vs basal; ∗P < 0.05 vs PA.
Natural Product Peptide α Amanitin, supplied by BioMimetic Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peptide+side-chain+mimetics/natural+product+peptide+%CE%B1+amanitin/pmc04573908-29-20-33
Average 90 stars, based on 1 article reviews
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angiotensin-converting enzyme  (BioMimetic Therapeutics)
90
BioMimetic Therapeutics angiotensin-converting enzyme
Effects of linear and <t>cyclic</t> <t>peptides</t> in vitro. (A) Cell viability MTT assay in hepatocytes treated with 0.4 mM palmitic acid (PA) with/without peptides (S1, 59 μM; liPS, IC and ICNal, 25 μM). Medium with 10 % FBS was used as positive control. (B) Cell-based ELISA assay for STAT1 activation in hepatocytes stimulated with PA for 6 h in the presence of increasing concentrations of peptides. Values expressed as ratio P-STAT1/STAT1 versus PA alone. (C) NOX-dependent measurement of O2•− in hepatocytes after 16 h of stimulation, assessed by chemiluminescence assay. BSA and apocynin were used as controls. Lucigenin relative units are expressed as fold increases versus basal conditions. (D – F) Expression levels of redox, cell stress, lipid metabolism, and inflammatory genes in hepatocytes pretreated with peptides (S1, 59 μM; liPS, 25 μM; IC/ICNal, 12 and 25 μM) before stimulation with PA for 6 h (D) and 24 h (E and F) . (G) ELISA analysis of cytokine concentrations in hepatocyte conditioned media after 24 h of stimulation. Gene expression of M1 (H) and M2 (I) markers in macrophages incubated for 24 h with conditioned media from hepatocytes stimulated with PA with/without peptides. The qPCR values normalized by 18S are expressed as fold increases versus basal conditions. Results are represented as individual data points and/or mean ± SD of n = 4–6 independent experiments. # P < 0.05 vs basal; ∗P < 0.05 vs PA.
Angiotensin Converting Enzyme, supplied by BioMimetic Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peptide+side-chain+mimetics/angiotensin+converting+enzyme/pm32180303-26-1-63
Average 90 stars, based on 1 article reviews
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chembiochem 2011, 12, 2025 – 2032  (Verlag GmbH)
90
Verlag GmbH chembiochem 2011, 12, 2025 – 2032
Effects of linear and <t>cyclic</t> <t>peptides</t> in vitro. (A) Cell viability MTT assay in hepatocytes treated with 0.4 mM palmitic acid (PA) with/without peptides (S1, 59 μM; liPS, IC and ICNal, 25 μM). Medium with 10 % FBS was used as positive control. (B) Cell-based ELISA assay for STAT1 activation in hepatocytes stimulated with PA for 6 h in the presence of increasing concentrations of peptides. Values expressed as ratio P-STAT1/STAT1 versus PA alone. (C) NOX-dependent measurement of O2•− in hepatocytes after 16 h of stimulation, assessed by chemiluminescence assay. BSA and apocynin were used as controls. Lucigenin relative units are expressed as fold increases versus basal conditions. (D – F) Expression levels of redox, cell stress, lipid metabolism, and inflammatory genes in hepatocytes pretreated with peptides (S1, 59 μM; liPS, 25 μM; IC/ICNal, 12 and 25 μM) before stimulation with PA for 6 h (D) and 24 h (E and F) . (G) ELISA analysis of cytokine concentrations in hepatocyte conditioned media after 24 h of stimulation. Gene expression of M1 (H) and M2 (I) markers in macrophages incubated for 24 h with conditioned media from hepatocytes stimulated with PA with/without peptides. The qPCR values normalized by 18S are expressed as fold increases versus basal conditions. Results are represented as individual data points and/or mean ± SD of n = 4–6 independent experiments. # P < 0.05 vs basal; ∗P < 0.05 vs PA.
Chembiochem 2011, 12, 2025 – 2032, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peptide+side-chain+mimetics/chembiochem+2016++17++1+++9/10__1002_slash_cbic__201100314-96-46-54
Average 90 stars, based on 1 article reviews
chembiochem 2011, 12, 2025 – 2032 - by Bioz Stars, 2026-09
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triscysteine peptide  (BioMimetic Therapeutics)
90
BioMimetic Therapeutics triscysteine peptide
Effects of linear and <t>cyclic</t> <t>peptides</t> in vitro. (A) Cell viability MTT assay in hepatocytes treated with 0.4 mM palmitic acid (PA) with/without peptides (S1, 59 μM; liPS, IC and ICNal, 25 μM). Medium with 10 % FBS was used as positive control. (B) Cell-based ELISA assay for STAT1 activation in hepatocytes stimulated with PA for 6 h in the presence of increasing concentrations of peptides. Values expressed as ratio P-STAT1/STAT1 versus PA alone. (C) NOX-dependent measurement of O2•− in hepatocytes after 16 h of stimulation, assessed by chemiluminescence assay. BSA and apocynin were used as controls. Lucigenin relative units are expressed as fold increases versus basal conditions. (D – F) Expression levels of redox, cell stress, lipid metabolism, and inflammatory genes in hepatocytes pretreated with peptides (S1, 59 μM; liPS, 25 μM; IC/ICNal, 12 and 25 μM) before stimulation with PA for 6 h (D) and 24 h (E and F) . (G) ELISA analysis of cytokine concentrations in hepatocyte conditioned media after 24 h of stimulation. Gene expression of M1 (H) and M2 (I) markers in macrophages incubated for 24 h with conditioned media from hepatocytes stimulated with PA with/without peptides. The qPCR values normalized by 18S are expressed as fold increases versus basal conditions. Results are represented as individual data points and/or mean ± SD of n = 4–6 independent experiments. # P < 0.05 vs basal; ∗P < 0.05 vs PA.
Triscysteine Peptide, supplied by BioMimetic Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peptide+side-chain+mimetics/triscysteine+peptide/pm29443519-62-9-3
Average 90 stars, based on 1 article reviews
triscysteine peptide - by Bioz Stars, 2026-09
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peptide for atx1  (BioMimetic Therapeutics)
90
BioMimetic Therapeutics peptide for atx1
Effects of linear and <t>cyclic</t> <t>peptides</t> in vitro. (A) Cell viability MTT assay in hepatocytes treated with 0.4 mM palmitic acid (PA) with/without peptides (S1, 59 μM; liPS, IC and ICNal, 25 μM). Medium with 10 % FBS was used as positive control. (B) Cell-based ELISA assay for STAT1 activation in hepatocytes stimulated with PA for 6 h in the presence of increasing concentrations of peptides. Values expressed as ratio P-STAT1/STAT1 versus PA alone. (C) NOX-dependent measurement of O2•− in hepatocytes after 16 h of stimulation, assessed by chemiluminescence assay. BSA and apocynin were used as controls. Lucigenin relative units are expressed as fold increases versus basal conditions. (D – F) Expression levels of redox, cell stress, lipid metabolism, and inflammatory genes in hepatocytes pretreated with peptides (S1, 59 μM; liPS, 25 μM; IC/ICNal, 12 and 25 μM) before stimulation with PA for 6 h (D) and 24 h (E and F) . (G) ELISA analysis of cytokine concentrations in hepatocyte conditioned media after 24 h of stimulation. Gene expression of M1 (H) and M2 (I) markers in macrophages incubated for 24 h with conditioned media from hepatocytes stimulated with PA with/without peptides. The qPCR values normalized by 18S are expressed as fold increases versus basal conditions. Results are represented as individual data points and/or mean ± SD of n = 4–6 independent experiments. # P < 0.05 vs basal; ∗P < 0.05 vs PA.
Peptide For Atx1, supplied by BioMimetic Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peptide+side-chain+mimetics/peptide+for+atx1/pm29443519-62-6-3
Average 90 stars, based on 1 article reviews
peptide for atx1 - by Bioz Stars, 2026-09
90/100 stars
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n-to-c peptide growth  (BioMimetic Therapeutics)
90
BioMimetic Therapeutics n-to-c peptide growth
Effects of linear and <t>cyclic</t> <t>peptides</t> in vitro. (A) Cell viability MTT assay in hepatocytes treated with 0.4 mM palmitic acid (PA) with/without peptides (S1, 59 μM; liPS, IC and ICNal, 25 μM). Medium with 10 % FBS was used as positive control. (B) Cell-based ELISA assay for STAT1 activation in hepatocytes stimulated with PA for 6 h in the presence of increasing concentrations of peptides. Values expressed as ratio P-STAT1/STAT1 versus PA alone. (C) NOX-dependent measurement of O2•− in hepatocytes after 16 h of stimulation, assessed by chemiluminescence assay. BSA and apocynin were used as controls. Lucigenin relative units are expressed as fold increases versus basal conditions. (D – F) Expression levels of redox, cell stress, lipid metabolism, and inflammatory genes in hepatocytes pretreated with peptides (S1, 59 μM; liPS, 25 μM; IC/ICNal, 12 and 25 μM) before stimulation with PA for 6 h (D) and 24 h (E and F) . (G) ELISA analysis of cytokine concentrations in hepatocyte conditioned media after 24 h of stimulation. Gene expression of M1 (H) and M2 (I) markers in macrophages incubated for 24 h with conditioned media from hepatocytes stimulated with PA with/without peptides. The qPCR values normalized by 18S are expressed as fold increases versus basal conditions. Results are represented as individual data points and/or mean ± SD of n = 4–6 independent experiments. # P < 0.05 vs basal; ∗P < 0.05 vs PA.
N To C Peptide Growth, supplied by BioMimetic Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peptide+side-chain+mimetics/n++to++c+peptide+growth/pm36700882-44-18-18
Average 90 stars, based on 1 article reviews
n-to-c peptide growth - by Bioz Stars, 2026-09
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biomimetic lipid/polymer assembly  (BioMimetic Therapeutics)
90
BioMimetic Therapeutics biomimetic lipid/polymer assembly
Effects of linear and <t>cyclic</t> <t>peptides</t> in vitro. (A) Cell viability MTT assay in hepatocytes treated with 0.4 mM palmitic acid (PA) with/without peptides (S1, 59 μM; liPS, IC and ICNal, 25 μM). Medium with 10 % FBS was used as positive control. (B) Cell-based ELISA assay for STAT1 activation in hepatocytes stimulated with PA for 6 h in the presence of increasing concentrations of peptides. Values expressed as ratio P-STAT1/STAT1 versus PA alone. (C) NOX-dependent measurement of O2•− in hepatocytes after 16 h of stimulation, assessed by chemiluminescence assay. BSA and apocynin were used as controls. Lucigenin relative units are expressed as fold increases versus basal conditions. (D – F) Expression levels of redox, cell stress, lipid metabolism, and inflammatory genes in hepatocytes pretreated with peptides (S1, 59 μM; liPS, 25 μM; IC/ICNal, 12 and 25 μM) before stimulation with PA for 6 h (D) and 24 h (E and F) . (G) ELISA analysis of cytokine concentrations in hepatocyte conditioned media after 24 h of stimulation. Gene expression of M1 (H) and M2 (I) markers in macrophages incubated for 24 h with conditioned media from hepatocytes stimulated with PA with/without peptides. The qPCR values normalized by 18S are expressed as fold increases versus basal conditions. Results are represented as individual data points and/or mean ± SD of n = 4–6 independent experiments. # P < 0.05 vs basal; ∗P < 0.05 vs PA.
Biomimetic Lipid/Polymer Assembly, supplied by BioMimetic Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peptide+side-chain+mimetics/biomimetic+assembly/10__1042_slash_bj20030784-210-32-32
Average 90 stars, based on 1 article reviews
biomimetic lipid/polymer assembly - by Bioz Stars, 2026-09
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tetrahydrobenzothiazole arginine side chain mimetics 1sb2 high resolution structure determination  (Mimetics)
86
Mimetics tetrahydrobenzothiazole arginine side chain mimetics 1sb2 high resolution structure determination
Effects of linear and <t>cyclic</t> <t>peptides</t> in vitro. (A) Cell viability MTT assay in hepatocytes treated with 0.4 mM palmitic acid (PA) with/without peptides (S1, 59 μM; liPS, IC and ICNal, 25 μM). Medium with 10 % FBS was used as positive control. (B) Cell-based ELISA assay for STAT1 activation in hepatocytes stimulated with PA for 6 h in the presence of increasing concentrations of peptides. Values expressed as ratio P-STAT1/STAT1 versus PA alone. (C) NOX-dependent measurement of O2•− in hepatocytes after 16 h of stimulation, assessed by chemiluminescence assay. BSA and apocynin were used as controls. Lucigenin relative units are expressed as fold increases versus basal conditions. (D – F) Expression levels of redox, cell stress, lipid metabolism, and inflammatory genes in hepatocytes pretreated with peptides (S1, 59 μM; liPS, 25 μM; IC/ICNal, 12 and 25 μM) before stimulation with PA for 6 h (D) and 24 h (E and F) . (G) ELISA analysis of cytokine concentrations in hepatocyte conditioned media after 24 h of stimulation. Gene expression of M1 (H) and M2 (I) markers in macrophages incubated for 24 h with conditioned media from hepatocytes stimulated with PA with/without peptides. The qPCR values normalized by 18S are expressed as fold increases versus basal conditions. Results are represented as individual data points and/or mean ± SD of n = 4–6 independent experiments. # P < 0.05 vs basal; ∗P < 0.05 vs PA.
Tetrahydrobenzothiazole Arginine Side Chain Mimetics 1sb2 High Resolution Structure Determination, supplied by Mimetics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peptide+side-chain+mimetics/1sb2+6+7+arginine+chain+determination+high+mimetics+resolution+side+structure+tetrahydrobenzothiazole/pm20712375__cb1001747_si_001-292-105-109
Average 86 stars, based on 1 article reviews
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polyisocyanide with peptide side chains  (BioMimetic Therapeutics)
90
BioMimetic Therapeutics polyisocyanide with peptide side chains
Effects of linear and <t>cyclic</t> <t>peptides</t> in vitro. (A) Cell viability MTT assay in hepatocytes treated with 0.4 mM palmitic acid (PA) with/without peptides (S1, 59 μM; liPS, IC and ICNal, 25 μM). Medium with 10 % FBS was used as positive control. (B) Cell-based ELISA assay for STAT1 activation in hepatocytes stimulated with PA for 6 h in the presence of increasing concentrations of peptides. Values expressed as ratio P-STAT1/STAT1 versus PA alone. (C) NOX-dependent measurement of O2•− in hepatocytes after 16 h of stimulation, assessed by chemiluminescence assay. BSA and apocynin were used as controls. Lucigenin relative units are expressed as fold increases versus basal conditions. (D – F) Expression levels of redox, cell stress, lipid metabolism, and inflammatory genes in hepatocytes pretreated with peptides (S1, 59 μM; liPS, 25 μM; IC/ICNal, 12 and 25 μM) before stimulation with PA for 6 h (D) and 24 h (E and F) . (G) ELISA analysis of cytokine concentrations in hepatocyte conditioned media after 24 h of stimulation. Gene expression of M1 (H) and M2 (I) markers in macrophages incubated for 24 h with conditioned media from hepatocytes stimulated with PA with/without peptides. The qPCR values normalized by 18S are expressed as fold increases versus basal conditions. Results are represented as individual data points and/or mean ± SD of n = 4–6 independent experiments. # P < 0.05 vs basal; ∗P < 0.05 vs PA.
Polyisocyanide With Peptide Side Chains, supplied by BioMimetic Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peptide+side-chain+mimetics/polyisocyanide+with+peptide+side+chains/10__1038_slash_s41570___019___0117___z-217-12-21
Average 90 stars, based on 1 article reviews
polyisocyanide with peptide side chains - by Bioz Stars, 2026-09
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Image Search Results


Effects of linear and cyclic peptides in vitro. (A) Cell viability MTT assay in hepatocytes treated with 0.4 mM palmitic acid (PA) with/without peptides (S1, 59 μM; liPS, IC and ICNal, 25 μM). Medium with 10 % FBS was used as positive control. (B) Cell-based ELISA assay for STAT1 activation in hepatocytes stimulated with PA for 6 h in the presence of increasing concentrations of peptides. Values expressed as ratio P-STAT1/STAT1 versus PA alone. (C) NOX-dependent measurement of O2•− in hepatocytes after 16 h of stimulation, assessed by chemiluminescence assay. BSA and apocynin were used as controls. Lucigenin relative units are expressed as fold increases versus basal conditions. (D – F) Expression levels of redox, cell stress, lipid metabolism, and inflammatory genes in hepatocytes pretreated with peptides (S1, 59 μM; liPS, 25 μM; IC/ICNal, 12 and 25 μM) before stimulation with PA for 6 h (D) and 24 h (E and F) . (G) ELISA analysis of cytokine concentrations in hepatocyte conditioned media after 24 h of stimulation. Gene expression of M1 (H) and M2 (I) markers in macrophages incubated for 24 h with conditioned media from hepatocytes stimulated with PA with/without peptides. The qPCR values normalized by 18S are expressed as fold increases versus basal conditions. Results are represented as individual data points and/or mean ± SD of n = 4–6 independent experiments. # P < 0.05 vs basal; ∗P < 0.05 vs PA.

Journal: Redox Biology

Article Title: Development of SOCS1 mimetics as novel approach to harmonize inflammation, oxidative stress, and fibrogenesis in metabolic dysfunction-associated steatotic liver disease

doi: 10.1016/j.redox.2025.103670

Figure Lengend Snippet: Effects of linear and cyclic peptides in vitro. (A) Cell viability MTT assay in hepatocytes treated with 0.4 mM palmitic acid (PA) with/without peptides (S1, 59 μM; liPS, IC and ICNal, 25 μM). Medium with 10 % FBS was used as positive control. (B) Cell-based ELISA assay for STAT1 activation in hepatocytes stimulated with PA for 6 h in the presence of increasing concentrations of peptides. Values expressed as ratio P-STAT1/STAT1 versus PA alone. (C) NOX-dependent measurement of O2•− in hepatocytes after 16 h of stimulation, assessed by chemiluminescence assay. BSA and apocynin were used as controls. Lucigenin relative units are expressed as fold increases versus basal conditions. (D – F) Expression levels of redox, cell stress, lipid metabolism, and inflammatory genes in hepatocytes pretreated with peptides (S1, 59 μM; liPS, 25 μM; IC/ICNal, 12 and 25 μM) before stimulation with PA for 6 h (D) and 24 h (E and F) . (G) ELISA analysis of cytokine concentrations in hepatocyte conditioned media after 24 h of stimulation. Gene expression of M1 (H) and M2 (I) markers in macrophages incubated for 24 h with conditioned media from hepatocytes stimulated with PA with/without peptides. The qPCR values normalized by 18S are expressed as fold increases versus basal conditions. Results are represented as individual data points and/or mean ± SD of n = 4–6 independent experiments. # P < 0.05 vs basal; ∗P < 0.05 vs PA.

Article Snippet: Lead compound S1, derived from SOCS1 KIR region (53–68), and new mimetics comprising a linear sequence with acetaminomethyl-cysteine substitution (liPS) and two cyclopeptides with a side-chain lactam bridge and additional 3-(1-naphthyl)-alanine substitution (IC and ICNal) were synthesized (ProteoGenix, Schiltigheim, France; KareBay Biochem, Monmouth Junction, NJ, USA) and structural characterized as previously reported [ , , ].

Techniques: In Vitro, MTT Assay, Positive Control, In-Cell ELISA, Activation Assay, Chemiluminescence Immunoassay, Expressing, Enzyme-linked Immunosorbent Assay, Gene Expression, Incubation